Dutch title: Endocriene verstoring in het Schelde-estuarium: distributie, blootstelling en effecten
Funder identifier: EV/22 (Other contract id)
Acronym: ENDIS-RISKS
Period: February 2002 till April 2006
Status: Completed
Thesaurus terms Hormones; Pesticides; Pollution
Geographical terms: ANE, Netherlands, Westerschelde [Marine Regions]; Belgium, Zeeschelde [Marine Regions]
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| Institutes (7) |
Top | Publications | Datasets |
- Universiteit Gent; Faculteit Bio-ingenieurswetenschappen; Vakgroep Toegepaste ecologie en milieubiologie; Laboratorium voor Milieutoxicologie en aquatische ecologie; Onderzoeksgroep voor Milieutoxicologie (ECOTOX), more, co-ordinator
- Universiteit Gent; Faculteit Wetenschappen; Vakgroep Biologie; Onderzoeksgroep Mariene Biologie (MARBIOL), more
- Koninklijk Belgisch Instituut voor Natuurwetenschappen; Departement Beheer van het Mariene Ecosysteem; Beheerseenheid Mathematisch Model Noordzee en Schelde-estuarium; Oostende (BMM), more
- Universiteit Gent; Faculteit Diergeneeskunde; Vakgroep Veterinaire Volksgezondheid & Voedselveiligheid; Laboratorium voor Chemische Analyse (LCA), more
- Rijksinstituut voor Kust en Zee (RIKZ), more
- Vlaams Instituut voor de Zee (VLIZ), more
- Belgian Science Policy (BELSPO), more, sponsor
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| Abstract |
- Context
There is growing concern about the potential of various chemicals to produce changes in the functioning of the endocrine system of humans and animals. Known natural hormones as well as relatively unknown environmental pollutants seem to have the ability to potentially disrupt the endocrine system of man and animal in such a way that harmful effects on their development and reproduction can occur.
The first indications of possible effects of endocrine disrupting substances and the presence of these substances in the Scheldt estuary have recently been published. The industrial areas of Ghent and Antwerp are to a large extent responsible for this pollution. Therefore, there is an obvious need to investigate the occurrence of endocrine disruption in the Scheldt estuary. In addition, a detailed knowledge of the distribution and long-term effects of these substances is needed in the framework of future-oriented policy measures at the national and European level.
- Project description
- Objectives
The major objectives within the project are:
- analyses of endocrine disrupters in water, suspended solids, sediment and biota (mysids, gobies);
- in vitro evaluation of the estrogenic and androgenic potency of samples of water, suspended solids and sediment;
- ecotoxicological and in situ evaluation of the effects on the resident mysid populations (short, intermediate and long-term);
- risk assessment of endocrine disrupters in the Scheldt estuary.
- Methodology
The proposed project can be divided into four different research phases:
Phase I: Distribution of endocrine disrupting substances in the Scheldt estuary (chemical analyses, analyses on biota, in vitro analyses)
The Scheldt estuary will be sampled three times a year (spring, summer and winter) for the duration of the project. On each sampling date, sediment, water, suspended solid and biota (mysid shrimp and gobies) will be collected. An extended list of natural hormones and endocrine disrupting chemicals will be measured in these samples.
The different water, sediment and suspended solid extracts will be tested in vitro to assess their potential to bind with the (human) estrogen and androgen receptor. The results of these analyses will allow an identification of the estrogenic and androgenic potency in different environmental matrices of the Scheldt estuary.
Phase II: Evaluation of the exposure of biota from the Scheldt estuary to endocrine disrupting substances
Based on the results of the first sampling campaigns priority substances will be selected. These priority endocrine-disrupting chemicals will be used in laboratory experiments with mysid shrimp to assess acute and chronic effects (Phase III).
Phase III: Ecotoxicological evaluation of the effects of endocrine disrupting substances occurring in the Scheldt estuary on resident mysid shrimp populations (laboratory and field studies)
Acute and chronic laboratory tests with mysid shrimps
To evaluate the possible effects of the pollutants (retained in phase II) on mysid shrimp, acute and chronic test will be performed in the laboratory. Mysid shrimp will also be exposed in multi-generation experiments during two generations (until the first generation of juveniles produces juveniles again) to assess the effects of endocrine disrupters during prolonged exposure.
Population study of the native mysid shrimp in the Scheldt estuary
The mysid shrimp population of the Scheldt estuary will be sampled and studied during one year. In this way, the present population dynamics can be compared with the available historical data and possible changes can be detected. In addition, a more in-depth field study on the possible endocrine disruption effects will be conducted based on the results of the laboratory toxicity experiments. Sensitive endpoints identified in the laboratory experiments will be validated in situ on the resident mysid shrimp population in the Scheldt estuary.
Phase IV: Risk assessment
In the last phase, the results of the previous phases will be incorporated into an integrated risk assessment for the Scheldt estuary with reference to endocrine disrupting substances. Moreover, an overall risk assessment for the Scheldt estuary will be conducted with these data in correlation with environmental concentrations of the tested endocrine disrupters. Recommendations for sustainable management and priority substances will be developed.
The results of this study on "endocrine disruption in the Scheldt estuary: distribution, exposure and effects" will be used at different levels and in different ways.
In order to ensure (rapid) dissemination and valorisation of the obtained results, the following actions will be undertaken:
- all results will be published in peer-reviewed journals;
- transfer (and discussion) of the obtained knowledge and insights to national and international representatives of policy-making and policy-supporting governmental services. This discussion will offer an essential contribution to the international contractual engagements of Belgium;
- diffusion of data and results through scientific databases;
- interactive communication forum during the project (project website);
- the results of the project will be brought together in a book, which will include the datasets on CD-rom and the main research results.
The diffusion and valorisation of the information as described above will be developed and maintained by the Flemish Institute for the Sea (VLIZ) (subcontracted) under supervision of the co-ordinator.
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| Datasets (14) |
Top | Institutes | Publications |
- Fockedey, N.; Mees, J.; Marine Biology Section - Ugent; (2003): Mysid shrimp populations in the Scheldt estuary., more
- Ghekiere A., Verslycke T., De Smet L., Van Beeumen J., Janssen C.R.; Environmental toxicology research group - Ugent; (2004): Metabolism, reproduction and moulting as endpoints to study endocrine disruption of Neomysis integerin the Scheldt Estuary, more
- Ghekiere, A.; Environmental toxicology research group - Ugent; (2004): Yeast Estrogen Screen (YES) and Yeast Androgen Screen (YAS) on water samples of the Scheldt estuary, more
- Janssen, C.R.; Environmental toxicology research group - Ugent, Belgium; (2006): ENDIS-RISKS fysico-chemical measurements and hyperbenthos in the Scheldt river, 2002-2006., more
- Monteyne, E.; Roose, P.; (2007): Supporting SCTD data of the ENDIS-RISKS sampling campaigns on the Belgian Continental shelf., more
- Monteyne, E.; Roose, P.; (2007): Supporting meteo data of the ENDIS-RISKS sampling campaigns on the Belgian Continental Shelf, more
- Monteyne, E.; Roose, P.; Management Unit of the North Sea Mathematical Models - RBINS; (2003): Chemical analysis of PAK's, Organotin, PCB's, PBDE's and organochlorine pesticides as possible endocrine disruptors in Scheldt estuary., more
- Monteyne, E.; Roose, P.; Management Unit of the North Sea Mathematical Models - RBINS; (2003): Organic carbon and nitrogen determination in Scheldt estuary samples, more
- Noppe, H.; De Brabander, H.; De Wasch, K.; Laboratory for Chemical Analysis - Ugent, Belgium; (2006): Chemical analysis of estrogens, organochlorine and organonitrogen pesticides as possible endocrine disruptors Scheldt-estuary., more
- Ecological epibenthic data of the Scheldt estuary, more
- Ecological epibenthic data of the Scheldt estuary: ENDIS-RISKS data (2002-2005), more
- Ecological hyperbenthic data of the Scheldt estuary, more
- Ecological hyperbenthic data of the Scheldt estuary: ENDIS-RISKS data (2002-2005), more
- IMERS: Integrated Marine Environmental Samples and Readings, more
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| Publications (23) |
Top | Institutes | Datasets |
- Noppe, H. (2006). Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. PhD Thesis. Universiteit Gent. Faculteit Diergeneeskunde: Gent. ISBN 978-90-5864-100-7. 153 pp., more
- Noppe, H.; De Wasch, K.; Poelmans, S.; Van Hoof, N.; Verslycke, T.; Janssen, C.R.; De Brabander, H. (2006). Development and validation of an analytical method for detection of estrogens in water, in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 21-41, more
- Noppe, H.; De Wasch, K.; Poelmans, S.; Van Hoof, N.; Verslycke, T.; Janssen, C.R.; De Brabander, H. (2005). Development and validation of an analytical method for detection of estrogens in water. Anal. Bioanal. Chem. 382(1): 91-98. dx.doi.org/10.1007/s00216-005-3174-8, more
- Ghekiere, A.; Fenske, M.; Verslycke, T.; Tyler, C.; Janssen, C.R. (2005). Development of a quantitative enzyme-linked immunosorbent assay for vitellin in the mysid Neomysis integer (Crustacea: Mysidacea). Comp. Biochem. Physiol., Part A Physiol. 142(1): 43-49. dx.doi.org/10.1016/j.cbpa.2005.07.006, more
- Fockedey, N. (2005). Dieet en groei van Neomysis integer (Leach, 1814) (Crustacea, Mysidacea) = Diet and growth of Neomysis integer (Leach, 1814) (Crustacea, Mysidacea). PhD Thesis. Universiteit Gent. Vakgroep Biologie, sectie Mariene Biologie: Gent. X, 297 pp., more
- Noppe, H.; Ghekiere, A.; Verslycke, T.; De Wulf, E.; Verheyden, K.; Monteyne, E.; Polfliet, K.; Van Caeter, P.; Janssen, C.R.; De Brabander, H.F. (2006). Distribution and ecotoxicity of chlorotriazines in the Scheldt estuary (Belgium-Netherlands), in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 85-105, more
- Fockedey, N.; Ghekiere, A.; Bruwiere, S.; Janssen, C.R.; Vincx, M. (2005). Effect of salinity and temperature on the intra-marsupial development of the brackish water mysid Neomysis integer (Crustacea: Mysidacea), in: Fockedey, N. Dieet en groei van Neomysis integer (Leach, 1814) (Crustacea, Mysidacea) = Diet and growth of Neomysis integer (Leach, 1814) (Crustacea, Mysidacea). pp. 227-247, more
- Verslycke, T.; Janssen, C.R. (2002). Effects of a changing abiotic environment on the energy metabolism in the estuarine mysid shrimp Neomysis integer (Crustacea: Mysidacea). J. Exp. Mar. Biol. Ecol. 279(1-2): 61-72. dx.doi.org/10.1016/S0022-0981(02)00339-8, more
- Rappé, K.; Ghekiere, A.; Noppe, H.; Monteyne, E.; Fockedey, N.; Janssen, C.; De Brabander, H.; Roose, P.; Vincx, M. (2007). ENDIS-RISKS: endocrine disruption in the Scheldt estuary - a field study, in: Mees, J. et al. (Ed.) VLIZ Young Scientists' Day, Brugge, Belgium 2 March 2007: book of abstracts. VLIZ Special Publication, 39: pp. 53, more
- Verslycke, T. (2003). Endocrine disruption in the estuarine invertebrate Neomysis integer (Crustacea: Mysidacea) = Endocriene verstoring bij de estuariene invertebraat Neomysis integer (Crustacea: Mysidacea). PhD Thesis. Universiteit Gent: Gent. VII, 224 pp., more
- Janssen, C.R.; Ghekiere, A.; Verslycke, T.; Vincx, M.; Fockedey, N.; Rappé, K.; De Brabander, H.; Noppe, H.; Roose, P.; Monteyne, E.; Vethaak, D.; Mees, J.; Deneudt, K. (2007). Endocrine disruption in the Scheldt estuary distribution, exposure and effects (ENDIS-RISKS). Final report. Belgian Science Policy: Brussels. 106 pp., more
- Monteyne, E.; Roose, P.; Janssen, C.R. (2007). Endocrine disruptor levels in invertebrates from the Scheldt estuary: cause for concern? BMM: Oostende. 1 poster pp., more
- Verslycke, T.A.; Vethaak, A.D.; Arijs, K.; Janssen, C.R. (2005). Flame retardants, surfactants and organotins in sediment and mysid shrimp of the Scheldt estuary (The Netherlands). Environ. Pollut. 136(1): 19-31. http://dx.doi.org/10.1016/j.envpol.2004.12.008, more
- Verslycke, T.; Fockedey, N.; McKenney Jr., C.L.; Roast, S.D.; Jones, M.; Mees, J.; Janssen, C.R. (2005). Mysid crustaceans as potential test organisms for the evaluation of environmental endocrine disruption: a review, in: Fockedey, N. Dieet en groei van Neomysis integer (Leach, 1814) (Crustacea, Mysidacea) = Diet and growth of Neomysis integer (Leach, 1814) (Crustacea, Mysidacea). pp. 249-275, more
- Verslycke, T.; Fockedey, N.; McKenney Jr., C.L.; Roast, S.D.; Jones, M.; Mees, J.; Janssen, C.R. (2004). Mysid crustaceans as potential test organisms for the evaluation of environmental endocrine disruption: a review. Environ. Toxicol. Chem. 23(5): 1219-1234. https://doi.org/10.1897/03-332, more
- Noppe, H.; Verslycke, T.; De Wulf, E.; Verheyden, K.; Monteyne, E.; van Caeter, P.; Janssen, C.R.; De Brabander, H.F. (2006). Occurrence of estrogens in the Scheldt estuary: a 2-year survey, in: Noppe, H. Analytiek van hormoon verstorende stoffen in milieumatrices = Analytics of endocrine disrupting chemicals in environmental matrices. pp. 41-60, more
- Noppe, H.; Verslycke, T.; De Wulf, E.; Verheyden, K.; Monteyne, E.; van Caeter, P.; Janssen, C.R.; De Brabander, H.F. (2007). Occurrence of estrogens in the Scheldt estuary: a 2-year survey. Ecotoxicol. Environ. Saf. 66(1): 1-8. https://dx.doi.org/10.1016/j.ecoenv.2006.04.005, more
- Ghekiere, A.; Verslycke, T.; De Smet, L.; Van Beeumen, J.; Janssen, C.R. (2006). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea), in: Ghekiere, A. Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). pp. 24-35, more
- Ghekiere, A.; Verslycke, T.; De Smet, L.; Van Beeumen, J.; Janssen, C.R. (2004). Purification and characterization of vitellin from the estuarine mysid Neomysis integer (Crustacea: Mysidacea). Comp. Biochem. Physiol. 138(4): 427-433. https://dx.doi.org/10.1016/j.cbpb.2004.05.012, more
- Verslycke, T.; Ghekiere, A.; Janssen, C.R. (2004). Seasonal and spatial patterns in cellular energy allocation in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) of the Scheldt estuary (The Netherlands). J. Exp. Mar. Biol. Ecol. 306(2): 245-267. dx.doi.org/10.1016/j.jembe.2004.01.014, more
- Ghekiere, A. (2006). Studie van invertebraat-specifieke effecten van endocrien-verstorende stoffen in de estuariene aasgarnaal Neomysis integer (Leach, 1814) = Study of invertebrate-specific effects of endocrine disrupting chemicals in the estuarine mysid Neomysis integer (Leach, 1814). PhD Thesis. Universiteit Gent. Faculteit Bio-ingenieurswetenschappen: Gent. ISBN 90-5989-105-8. 143 pp., more
- Fockedey, N.; Mees, J.; Vangheluwe, M.; Verslycke, T.; Janssen, C.R.; Vincx, M. (2005). Temperature and salinity effects on post-marsupial growth of Neomysis integer (Crustacea: Mysidacea). J. Exp. Mar. Biol. Ecol. 326(1): 27-47. https://dx.doi.org/10.1016/j.jembe.2005.05.005, more
- Verslycke, T.; Poelmans, S.; De Wash, K.; Vercauteren, J.; Devos, C.; Moens, L.; Sandra, P.; De Brabander, H.F.; Janssen, C.R. (2003). Testosterone metabolism in the estuarine mysid Neomysis integer (Crustacea; Mysidacea) following tributyltin exposure. Environ. Toxicol. Chem. 22(9): 2030-2036, more
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